Transcription termination and late control in phage lambda.

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Transcription termination and late control in phage lambda.

A transcription termination site occurs between the promoter for late gene expression of bacteriophage lambda and the late genes themselves. It is proposed that the lambda Q gene product controls late gene expression by over-coming this termination barrier.

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Nun protein from coliphage HK022 binds to phage boxB RNA and functions, in contrast to phage lambda N protein, as a transcriptional terminator. The basic Nun-(10-44) peptide contains the boxB RNA binding arginine rich motif, ARM. The peptide binds boxB RNA and competes with the phage lambda ARM peptide N-(1-36) as indicated by nuclear magnetic resonance (NMR) spectroscopy titrations. In two-dim...

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*Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032; †Institute of Cancer Research, College of Physicians and Surgeons, Columbia University, New York, NY 10032; §Molecular Control and Genetics Section, Gene Regulation and Chromosome Biology, National Cancer Institute, National Institutes of Health, Frederick, MD 21702...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1975

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.72.9.3300